{"created":"2023-05-15T08:35:30.725468+00:00","id":281,"links":{},"metadata":{"_buckets":{"deposit":"4eb8ca70-3614-46ef-ae2c-6982e98340f5"},"_deposit":{"created_by":1,"id":"281","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"281"},"status":"published"},"_oai":{"id":"oai:tsukuba-tech.repo.nii.ac.jp:00000281","sets":["6:7:16"]},"author_link":["12625"],"control_number":"281","item_2_alternative_title_21":{"attribute_name":"タイトルヨミ","attribute_value_mlt":[{"subitem_alternative_title":"マイクロ　パターン　セッチャクメン　ジョウ　デ　バイヨウシタ　センイガ　サイボウ　ノ　サイボウ　ジク　ケッテイ　キコウ","subitem_alternative_title_language":"ja"}]},"item_2_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"12","bibliographicPageStart":"6","bibliographicVolumeNumber":"23","bibliographic_titles":[{"bibliographic_title":"筑波技術大学テクノレポート"}]}]},"item_2_date_64":{"attribute_name":"最初の登録日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2015-12-24"}]},"item_2_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"線維芽細胞の基底部に局在する接着斑（focal adhesion）は細胞の接着，移動，創傷の\n治癒に重要な役割を演じている構造である。Focal adhesion は細胞膜と細胞外基質の状態を認識し，極性を決定する機能を持っていると考えられる。しかしながら，線維芽細胞が基底面に接着する初期段階における細胞外基質と細胞内の認識メカニズムと極性の形成機構についてはほとんど明らかになっていない。今回，著者はごく狭い幅で細胞接着を制御できるマイクロパターン培養ガラス（Cytograph; 10, 15, 30, 60 μm 幅）を用い，接着初期とその後の細胞軸形成時のfocal\nadhesion の変化を，チロシンリン酸化タンパク質とfocal adhesion kinase （FAK）との関わりを中心\nに観察を進めた。線維芽細胞をマイクロパターン上に培養すると，まず接着面において小さいfocal\nadhesion 様構造が形成され，やがて細胞は接着面と非接着領域の境界部に沿って紡錘状に伸長を始めた。伸長しつつある細胞の先端部位では多数の小さい focal adhesion 様構造が，また，境界部では良く成熟したfocal adhesion が形成された。さらに，境界部では細胞の長軸方向に沿ってFAK の集積が観察され，FAK が細胞軸の決定に重要であることが示唆された。","subitem_description_type":"Abstract"}]},"item_2_publisher_36":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"筑波技術大学学術・社会貢献推進委員会"}]},"item_2_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA12123772","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2435-4856","subitem_source_identifier_type":"EISSN"}]},"item_2_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"筑波技術大学　保健科学部　保健学科　鍼灸学専攻"}]},"item_2_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"加藤, 一夫","creatorNameLang":"ja"},{"creatorName":"カトウ, カヅオ","creatorNameLang":"ja-Kana"},{"creatorName":"Katoh, Kazuo","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-02-26"}],"displaytype":"detail","filename":"Tec23_1_02.pdf","filesize":[{"value":"1.9 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Tec23_1_02.pdf","url":"https://tsukuba-tech.repo.nii.ac.jp/record/281/files/Tec23_1_02.pdf"},"version_id":"776120f8-697d-4f81-bb2e-ad19c653ea67"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-02-26"}],"displaytype":"detail","filename":"Tec23_1_02.txt","filesize":[{"value":"14.2 kB"}],"format":"text/plain","licensetype":"license_note","mimetype":"text/plain","url":{"label":"Tec23_1_02.txt","url":"https://tsukuba-tech.repo.nii.ac.jp/record/281/files/Tec23_1_02.txt"},"version_id":"8812c9d8-fcac-421a-bdee-56c86d68672e"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"細胞接着","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"マイクロパターン接着面上で培養した線維芽細胞の細胞軸決定機構","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"マイクロパターン接着面上で培養した線維芽細胞の細胞軸決定機構","subitem_title_language":"ja"}]},"item_type_id":"2","owner":"1","path":["16"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2015-12-24"},"publish_date":"2015-12-24","publish_status":"0","recid":"281","relation_version_is_last":true,"title":["マイクロパターン接着面上で培養した線維芽細胞の細胞軸決定機構"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2024-01-30T07:06:22.867836+00:00"}