@article{oai:tsukuba-tech.repo.nii.ac.jp:00001255,
 author = {殿山, 希 and Donoyama, Nozomi and 和久田, 哲司 and Wakuda, Tetsuji and 谷津, 忠志 and Tanitsu, Tadashi and 一幡, 良利 and Ichiman, Yoshitoshi},
 issue = {2},
 journal = {International Journal of Aromatherapy},
 month = {},
 note = {Objective: The essential oil tea tree oil (TTO) possesses powerful antibacterial activity and many aromatherapists in Japan use it for preventing infection during foot massage. Thus, whether massage is more hygienic using oil with added TTO was evaluated.
Design: Massage sessions were conducted using different oils as lubricant; 3% TTO (TTO diluted to 3% v/v with Jojoba oil base), 6% TTO, 3% lavender oil (LO), 6% LO and Jojoba oil without any essential oil added as control. Samples were taken from the therapist's palms and client's skin, then surviving bacteria were counted. Also, to clarify the growth inhibition effect on bacteria, 4.80×103 colony forming units (CFU) of Staphylococcus aureus was added to the 5 kinds of oils used as lubricants, undiluted TTO, and undiluted LO, and reduction of bacterial survival count measured.
Results: Bacterial count on the therapist's palms increased and that on the client's skin decreased after 10-min. and 20-min. massage. Bacterial count was dependent on concentration of essential oils, however this tendency was not special on TTO. Using undiluted TTO and LO in bacterial growth experiments, no multiplication of the organisms was observed, but this effect was not complete using diluted TTO and LO.
Conclusion: Undiluted TTO had antibacterial activity against S. aureus in vitro, however, it could not be demonstrated that it was effective to use the oil added TTO as lubricant for the purpose of hygienic massage practice., International Journal of Aromatherapy},
 pages = {106--109},
 title = {Using tea tree oil for hygienic massage practice},
 volume = {15},
 year = {2005},
 yomi = {ドノヤマ, ノゾミ and ワクダ, テツジ and タニツ, タダシ and イチマン, ヨシトシ}
}